Radio Astronomy

Contains reports on five research projects.National Science Foundation (Grant AST82-14296)National Aeronautics and Space Administration (Grant NAG W-373)National Aeronautics and Space Administration (Grant NAG5-537)U.S. Navy - Office of Naval Research (Contract N00014-84-C-2082)SM Systems and Research, Inc.Defense Advanced Research Project Agency (Contract MDA903-82-K-0521

Radio Astronomy

Contains summary of research and reports on seven research projects.National Science Foundation (Grant AST82-14296)National Aeronautics and Space Administration (Grant NAGW-373)National Aeronautics and Space Administration (Contract NAS5-28410)U.S. Navy - Office of Naval Research (Contract N00014-84-C-2082)M.I.T. Sloan Fund for Basic ResearchNational Oceanic and Atmospheric Administration (Grant 04-8-M01-1)National Aeronautics and Space Administration (Grant NAG5-10)Defense Advanced Research Project Agency (Contract MDA 903-84-K-0297

Radio Astronomy

Contains summary of research and reports on nine research projects.National Science Foundation (Grant AST81-21416)National Science Foundation (Grant AST82-14296)National Aeronautics and Space Administration (Grant S-10781-C)National Aeronautics and Space Administration (Grant NAGW-373)National Science Foundation (Grant AST79-19553)M.I.T. Sloan Fund for Basic ResearchNational Oceanic and Atmospheric Administration (Grant 04-8-M01-1)National Aeronautics and Space Administration (Grant NAG5-10)National Aeronautics and Space Administration (Contract NAS5-22929)Defense Advanced Research Projects Agency (Contract MDA 903-82-K-0521)Center for Advanced Television Studie

Expression of the alpha7 isoform of hepatocyte nuclear factor (HNF) 4 is activated by HNF6/OC-2 and HNF1 and repressed by HNF4alpha1 in the liver

The hepatocyte nuclear factor (HNF) 4alpha gene possesses two promoters, proximal P1 and distal P2, whose use results in HNF4alpha1 and HNF4alpha7 transcripts, respectively. Both isoforms are expressed in the embryonic liver, whereas HNF4alpha1 is almost exclusively in the adult liver. A 516-bp fragment, encompassing a DNase I-hypersensitive site associated with P2 activity that is still retained in adult liver, contains functional HNF1 and HNF6 binding sites and confers full promoter activity in transient transfections. We demonstrate a critical role of the Onecut factors in P2 regulation using site-directed mutagenesis and embryos doubly deficient for HNF6 and OC-2 that show reduced hepatic HNF4alpha7 transcript levels. Transient transgenesis showed that a 4-kb promoter region is sufficient to drive expression of a reporter gene in the stomach, intestine, and pancreas, but not the liver, for which additional activating sequences may be required. Quantitative PCR analysis revealed that throughout liver development HNF4alpha7 transcripts are lower than those of HNF4alpha1. HNF4alpha1 represses P2 activity in transfection assays and as deduced from an increase in P2-derived transcript levels in recombinant mice in which HNF4alpha1 has been deleted and replaced by HNF4alpha7. We conclude that although HNF6/OC-2 and perhaps HNF1 activate the P2 promoter in the embryo, increasing HNF4alpha1 expression throughout development causes a switch to essentially exclusive P1 promoter activity in the adult liver

PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments

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